The list of studies that can be performed at the Toxicology Research Group:
In vitro, ex vivo, in vivo eye corrosion/ irritation studies
Isolated chicken eye test method for identifying
I) chemicals inducing serious eye damage,
II) chemicals not requiring classification for eye irritation or serious eye damage.
Short time exposure in vitro test method for identifying I) chemicals inducing serious eye damage
II) chemicals not requiring classification for eye irritation or serious eye damage.
The test method used within the study on the model of reconstructed human cornea-like epithelium (RhCE), used for identifying chemicals not requiring classification and labelling for eye irritation or serious eye damage.
Reconstructed human cornea-like epithelium (RHCE) test method for eye hazard identification.
Acute eye irritation/ eye corrosion test.
In vitro, ex vivo, in vivo skin corrosion/ irritation studies
In vitro skin corrosion: transcutaneous electrical resistance test (TER).
In vitro skin corrosion: reconstructed human epidermis (RHE) test method.
In vitro skin irritation. The test on the model of reconstructed human epidermis.
Acute skin irritation/ corrosion test.
Biological evaluation of medical devices – Test for skin irritation in vitro.
ISO 10993-23
In vitro, in chemico, in vivo skin sensitisation studies
Skin sensitisation: local lymph node assay (LLNA): BrdU-ELISA.
In chemico skin sensitisation, direct peptide reactivity assay (DPRA).
In vitro skin sensitisation: the test method with use of ARE-Nrf2 Luciferase.
In vitro skin sensitisation: Human cell line activation test (H-CLAT).
Skin sensitisation study.
Delayed hypersensitivity assessment test using the closed-patch method (Buehler Test).
ISO 10993-10
In vitro cytotoxicity studies
In vitro cytotoxicity test.
In vitro phototoxicity
Phototoxicity – In vitro 3T3 NRU phototoxicity test.
In vitro Phototoxicity – Reconstructed Human Epidermis Phototoxicity test method.
In vitro Pyrogen Test
Monocyte Activation Test
ISO/ Farmakopea
In vitro, in vivo studies identifying endocrine-disrupting substances
Stably Transfected Human Estrogen Receptor-α Transactivation Assay for Detection of Estrogenic Agonist and antagonist Activity of Chemicals using the hERα-HeLa-9903 cell line.
Uterotrophic Bioassay in Rodents
A short-term screening test for oestrogenic properties.
Hershberger bioassay in rats. Short-term screening test of (anti-) androgenic properties.
H295R Steroidogenesis Assay
In vitro, in vivo acute toxicity
Use of the 3T3 neutral red uptake cytotoxicity test to estimate starting doses for acute oral systemic toxicity tests.
Acute oral toxicity test.
Acute oral/ intraperitoneal/ subcutaneous/ intravascular toxicity test – acute toxic class method
Acute dermal toxicity test.
Acute inhalation toxicity test.
Acute inhalation toxicity – acute toxic class method.
Maximum tolerated dose test (MTD).
Single dose toxicity study in rodents.
ISO 10993-11
In vivo subacute, subchronic and chronic studies
Dose range finding test (DRF).
Repeated dose 28-day oral/ intraperitoneal/ subcutaneous toxicity study in rodents.
Repeated dose dermal toxicity: 21/ 28 day study.
Repeated dose 90-day oral toxicity study in rodents.
Subchronic dermal toxicity: 90-day study.
Chronic toxicity studies.
Combined chronic toxicity/ carcinogenicity studies.
In vivo neurotoxicity studies
Neurotoxicity study in rodents.
In vivo developmental and reproduction studies
Prenatal developmental toxicity study.
Reproduction/ developmental toxicity screening test
Combined repeated dose toxicity study with the reproduction/ developmental toxicity screening test.
Extended one-generation reproductive toxicity study in rats.
Two-generation reproduction toxicity study.
In vitro, in vivo genotoxicity studies
Bacterial reverse mutation test (AMES test, microplate format).
In vitro micronucleus test on the mammalian cell line.
In vitro mammalian cell gene mutation tests using the HPRT genes.
In vivo mammalian erythrocyte micronucleus test.
In vivo mammalian cell gene mutation test using the thymidine kinase gene.
In Vivo Mammalian Alkaline Comet Assay.
In vivo carcinogenicity studies
Badanie rakotwórczości
Combined chronic toxicity/ carcinogenicity studies.
In vivo local tolerance studies
Local tolerance test.
In vivo local tolerance studies
Toxicokinetics/pharmacokinetics study.
OECD Nr 417/ EU B.436/ ICH S3A
Other services
Toxicological studies conducted according to the procedures agreed with the customer
Haematological studies
Biochemical studies
Hormone identification
Tissue and organ collection
Preparing histopathology slides and their scanning
List of studies that can be performed at the Ecotoxicology Research Group
Aquatic toxicity studies
Freshwater algae and cyanobacteria, growth inhibition test.
Lemna sp. growth inhibition test.
Sediment–free Myriophyllum spicatum toxicity test.
Water–sediment (Myriophyllum spicatum) macrophytes spicatum toxicity test.
Daphnia magna, acute immobilisation test.
Daphnia magna reproduction test.
Sediment–water chironomid toxicity using spiked sediment.
Sediment–water chironomid toxicity using spiked water.
Sediment–water chironomid life-cycle toxicity test using spiked water or spiked sediment.
Chironomus sp., acute immobilisation test.
Fish Cell Line Acute Toxicity – The RTgill-W1 cell line assay.
Fish embryo acute toxicity (FET) test.
Fish, acute toxicity test.
Fish, early-life stage toxicity test.
Fish, short-term toxicity test on embryo and sac-fry stages.
Fish, juvenile growth test.
Bioaccumulation in fish: aqueous and dietary exposure.
21-day fish assay: a short-term screening for oestrogenic and androgenic acitivity in zebrafish.
Toxicity studies for soil organisms
Terrestrial plant test, seedling emergence and seedling growth test.
Terrestrial plant test, vegetative vigour test.
Earthworm, acute toxicity test.
Earthworm, reproduction test. (Eisenia fetida/Eisenia andrei)
Collembolan, reproduction test in soil. (Folsomia candida)
Predatory mite Hypoaspis (Geolaelaps), reproduction test in soil.
Soil microorganisms: nitrogen transformation test.
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Soil microorganisms: carbon transformation test. |
Toxicity tests for pollinators and beneficial arthropods
Honeybees, acute oral toxicity test (Apis mellifera)
Honeybees, acute contact toxicity test (Apis mellifera)
Honeybees, larval toxicity test (Apis mellifera) – single exposure
Honeybees, larval toxicity test (Apis mellifera) – repeated exposure.
Honeybees (Apis mellifera), chronic oral toxicity test (10-day feeding).
Study of honeybees’ flight ability to return to the hive after a single oral exposure to sublethal doses of chemicals.
OECD Nr 332
Bumblebees (Bombus spp.), acute oral toxicity test.
Bumblebees (Bombus spp.), acute contact toxicity test.
A laboratory test or extended laboratory test for evaluating the effects of plant protection products on the parasitic wasp, Aphidius rhopalosiphi De Stephani-Perez.
SETAC; ESCORT 1, ESCORT 2; IOBC/BART/EPPO
A laboratory test or extended laboratory test for evaluating the effects of plant protection products on the predatory mite, Typhlodromus pyri Scheuten.
SETAC; ESCORT 1, ESCORT 2; IOBC/BART/EPPO
A laboratory test for evaluating the effects of plant protection products on the spider, Pardosa.
SETAC; ESCORT 1, ESCORT 2; IOBC/BART/EPPO
A laboratory test or extended laboratory test for evaluating the effects of plant protection products on the seven spotted ladybird beetle, Coccinella septempunctata L.
ESCORT 1, ESCORT 2, IOBC, BART, EPPO
A laboratory test or extended laboratory test for evaluating the effects of plant protection products on the green lacewing, Chrysoperla carnea.
ESCORT 1, ESCORT 2, IOBC, BART, EPPO
Aged residue test on the parasitic wasp, Aphidius rhopalosiphi De Stephani-Perez.
ESCORT 1, ESCORT 2, IOBC, BART, EPPO
Aged residue test on the predatory mite, Typhlodromus pyri Scheuten.
ESCORT 1, ESCORT 2, IOBC, BART, EPPO
Aged residue test on the seven spotted ladybird beetle, Coccinella septempunctata L.
ESCORT 1, ESCORT 2, IOBC, BART, EPPO
Aged residue test on the green lacewing, Chrysoperla carnea.
ESCORT 1, ESCORT 2, IOBC, BART, EPPO
A test for evaluating the chronic effects of plant protection products on the rove beetle Aleochara bilineata (Coleoptera: Staphylinidae)
SETAC; ESCORT 1, ESCORT 2; IOBC/BART/EPPO
A method for testing effects of plant protection products on the carabid beetle Poecilus cupreus (Coleoptera, Carabidae)
SETAC; ESCORT 1, ESCORT 2; IOBC/BART/EPPO
Honeybee (Apis mellifera L.) Homing Flight Test, using single oral exposure to sublethal doses of test chemical
Fate and behaviour in the environment
Ready biodegradability
OECD Nr 301D/ EU C.4-E;
OECD Nr 301F/ EU C.4-D
Inherent biodegradability: Zahn-Wellens/ EMPA test
Activated sludge, respiration inhibition test
Aerobic and anaerobic transformation in soil
Leaching in soil columns
Development and validation of an analytical method in water, soil, sediments and plant and animal materials
SANTE
Determination of the residues of chemical substances in water, soil, sedimemt, plant and animal materials
SANTE/2020/12830
Stability of pesticide residues in stored commodities
Bioaccumulation in Terrestrial Oligochaetes
Physical and chemical properties
Water solubility
Solubility in organic solvents
Adsorption – desorption using batch equilibrium method
Partition coefficient (n-octanol/water): shake flask method
Hydrolysis as a function of pH
Partition coefficient (n-octanol/water), HPLC method
Estimation of the adsorption coefficient (KOC) on soil and on sewage sludge using high performance liquid chromatography (HPLC)
Avian toxicology
Avian dietary toxicity test
Avian acute oral toxicity test
Avian repellency test (Coturnix coturnix japonica)
Ecosystem structure study
Simulated freshwater lentic field test (outdoor microcosms and mesocosms)
Contact:
Łukasiewicz – Institute of industrial Organic Chemistry
Branch in Pszczyna
ul. Doświadczalna 27, 43-200 Pszczyna
e-mail: sekretariat.pszczyna@ipo.lukasiewicz.gov.pl
tel: +48 32 210 30 81
