Tests
Certified | accredited research
Physical-chemical testing
We hold the Good Laboratory Practice Compliance Certificate No. 15/2023/GLP in the fields of: physical-chemical testing, studies on the behaviour of the tested substance in water, soil and air, bioaccumulation studies, residue studies (analytical phase), other studies: chemical analysis.
Determination of the density of liquids and solids
CIPAC MT 3
EC A.3
Determination of pH values
CIPAC MT 75.3
Acidity/alkalinity
CIPAC MT 191
Determination of kinematic viscosity and calculation of dynamic viscosity
Determination of apparent viscosity using the Brookfield method
OECD 114
Storage of chemical substances and chemical preparations at a controlled temperature of 20±2℃
GIFAP No. 17
Accelerated aging test
CIPAC MT 46.4.
Storage of chemical substances and preparations at low temperatures and assessment of their physical stability
CIPAC MT 39.3
Determination of durability of diluted plant protection products forming solutions with water
CIPAC MT 41.1
Pourability of suspension concentrates
CIPAC MT 148.1; 148
Determination of wettability
CIPAC MT 53.3
Methods of determining the stability of aqueous suspensions
CIPAC MT 184.1
Determination of the stability of emulsions and water suspo-emulsion
CIPAC MT 36.3; 180
Determination of foaming
CIPAC MT 47.3
Determination of bulk density loose and tapped
CIPAC MT 186
Grain size determination of granular materials by dry method
CPAC MT 170
Grain size determination by the wet method
CPAC MT 185
Determination of solubility of packaging in water
CPAC MT 176; 18
Packaging test
Crop Life Inernational. Technical Monograph No 17
Test of efficiency of packaging washing
Efficacy Guideline 305
Appearance and physical state
OPPTS od 830.6302 do 04
Determination of particle size distribution
OECD 110
CIPAC MT 187
Determination of the Spontaneity Dispersion
CIPAC MT 174; 160
Determination of Granular Product Dust Content
CIPAC MT 171.1
Determination of seed treatment distribution uniformity and adhesion
CIPAC MT 175
Attrition resistance and friability
CIPAC MT 178.2
Physical compatibility of pesticides
ASTM E1518
Determination of the adhesion agent on seeds
CIPAC MT 194, 175
Determination of surface tension of aqueous solutions
EC A. 5
Determination of solubility of chemicals substances
EC A. 6
Partition coefficient n-octanol/water: shake flask method
OECD 107
Determination of pour points
EC A.1
Determination of melting point of chemical substances
EC A.1
Determination of boiling point of chemical substances
EC A.2
Determination of organic carbon
Shimadzu Analyser
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Dorota Drążewska
e-mail: dorota.drazewska@ipo.lukasiewicz.gov.pl Telephon: +48 22 88 41 221
Physical-chemical testing
Determination of vapor pressures of substances using static method
OECD 104
Testing corrosive effects on metals: a test for determining the corrosive properties of liquids and solids that may become liquid during transport
Manual of Tests and Criteria, Seventh revised edition ST/SG/AC.10/11/Rev.7. United Nations, New York and Geneva, 2019 – Section 37 Classification procedures, test methods and criteria relating to substances corrosive to metals, Test C.1.
Determination of oxidising properties of solids
Test UN O.1
Determination of oxidizing properties of liquids
Test UN O.2
Determination of the auto-ignition temperature of substances (liquids and gases)
PN-EN ISO/IEC
80079-20-1:2020-03
Flammability – solid
Test UN N.1
Determination of flash point by the Pensky-Martens closed-cup method
PN-EN ISO 2719:2016
Determination of flash point by the Abel closed-cup method
PN-EN ISO 13736:2021
Determination of ignition or non-ignition and flash point temperature using a rapid equilibrium method in a closed crucible
PN-EN ISO 3679:2023
Determination of the boiling temperature of substances by DSC and TG methods
EC A.2
OECD 103
Determining specific heat by DSC method
OECD 114
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MSc Eng. Michał Frączak
e-mail:michal.fraczak@ipo.lukasiewicz.gov.pl Telephon: +48 22 88 41 350
Physical-chemical testing
Determination of explosive properties:
– determination of impact sensitivity
– determination of friction sensitivity
– determination of thermal sensitivity
EC A. 14
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Rafał Lewczuk, PhD
e-mail: rafal.lewczuk@ipo.lukasiewicz.gov.pl Telephon: +48 22 88 41 365
Analytical studies
Determination of active substance content and significant impurities using GC and HPLC techniques
SANCO/
3030/99 rev.5
Determination of substance content by potentiometric titration
Titroline apparatus
Determination of free phenols content
CIPAC MT 69.1-2
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Anna Kiełczewska, MSc Eng.
e-mail: anna.kielczewska@ipo.lukasiewicz.gov.pl
Telephon:
+48 22 88 41 295
Biodegradability test
Ready aerobic biodegradability screening test of substances in an aerobic aquatic environment using manometric respirometry
Ready aerobic biodegradability screening test of substances in an aerobic aquatic environment using the closed bottle method
OECD 301
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Dorota Drążewska
e-mail: dorota.drazewska@ipo.lukasiewicz.gov.pl Telephon: +48 22 88 41 221
Toxicological studies
The studies are conducted under the Good Laboratory Practice quality system (Certificate No. 10/2024/GLP) according to methods: OECD, EU, SCCS, ISO, EMA, FDA, and their results are used for registration purposes, licensing, as well as other regulations in the field of industrial chemicals.
We hold Good Laboratory Practice certificates and full accreditation from AAALAC International, granted by the Association for Assessment and Accreditation of Laboratory Animal Care (AAALAC) International Council. This accreditation confirms adherence to the highest standards in ensuring the welfare of laboratory animals.
In vitro, ex vivo, in vivo eye corrosion/ irritation studies
Isolated chicken eye test method for identifying
I) chemicals inducing serious eye damage,
II) chemicals not requiring classification for eye irritation or serious eye damage.
Short time exposure in vitro test method for identifying I) chemicals inducing serious eye damage
II) chemicals not requiring classification for eye irritation or serious eye damage.
The test method used within the study on the model of reconstructed human cornea-like epithelium (RhCE), used for identifying chemicals not requiring classification and labelling for eye irritation or serious eye damage.
Reconstructed human cornea-like epithelium (RHCE) test method for eye hazard identification.
Acute eye irritation/ eye corrosion test.
In vitro, ex vivo, in vivo skin corrosion/ irritation studies
In vitro skin corrosion: transcutaneous electrical resistance test (TER).
In vitro skin corrosion: reconstructed human epidermis (RHE) test method.
In vitro skin irritation. The test on the model of reconstructed human epidermis.
Acute skin irritation/ corrosion test.
Biological evaluation of medical devices – Test for skin irritation in vitro.
ISO 10993-23
In vitro, in chemico, in vivo skin sensitisation studies
Skin sensitisation: local lymph node assay (LLNA): BrdU-ELISA.
In chemico skin sensitisation, direct peptide reactivity assay (DPRA).
In vitro skin sensitisation: the test method with use of ARE-Nrf2 Luciferase.
In vitro skin sensitisation: Human cell line activation test (H-CLAT).
Skin sensitisation study.
Delayed hypersensitivity assessment test using the closed-patch method (Buehler Test).
ISO 10993-10
In vitro cytotoxicity studies
In vitro cytotoxicity test.
In vitro phototoxicity
Phototoxicity – In vitro 3T3 NRU phototoxicity test.
In vitro Phototoxicity – Reconstructed Human Epidermis Phototoxicity test method.
In vitro Pyrogen Test
Monocyte Activation Test
ISO/ Farmakopea
In vitro, in vivo studies identifying endocrine-disrupting substances
Stably Transfected Human Estrogen Receptor-α Transactivation Assay for Detection of Estrogenic Agonist and antagonist Activity of Chemicals using the hERα-HeLa-9903 cell line.
Uterotrophic Bioassay in Rodents
A short-term screening test for oestrogenic properties.
Hershberger bioassay in rats. Short-term screening test of (anti-) androgenic properties.
H295R Steroidogenesis Assay
In vitro, in vivo acute toxicity
Use of the 3T3 neutral red uptake cytotoxicity test to estimate starting doses for acute oral systemic toxicity tests.
Acute oral toxicity test.
Acute oral/ intraperitoneal/ subcutaneous/ intravascular toxicity test – acute toxic class method
Acute dermal toxicity test.
Acute inhalation toxicity test.
Acute inhalation toxicity – acute toxic class method.
Maximum tolerated dose test (MTD).
Single dose toxicity study in rodents.
ISO 10993-11
In vivo subacute, subchronic and chronic studies
Dose range finding test (DRF).
Repeated dose 28-day oral/ intraperitoneal/ subcutaneous toxicity study in rodents.
Repeated dose dermal toxicity: 21/ 28 day study.
Repeated dose 90-day oral toxicity study in rodents.
Subchronic dermal toxicity: 90-day study.
Chronic toxicity studies.
Combined chronic toxicity/ carcinogenicity studies.
In vivo neurotoxicity studies
Neurotoxicity study in rodents.
In vivo developmental and reproduction studies
Prenatal developmental toxicity study.
Reproduction/ developmental toxicity screening test
Combined repeated dose toxicity study with the reproduction/ developmental toxicity screening test.
Extended one-generation reproductive toxicity study in rats.
Two-generation reproduction toxicity study.
In vitro, in vivo genotoxicity studies
Bacterial reverse mutation test (AMES test, microplate format).
In vitro micronucleus test on the mammalian cell line.
In vitro mammalian cell gene mutation tests using the HPRT genes.
In vivo mammalian erythrocyte micronucleus test.
In vivo mammalian cell gene mutation test using the thymidine kinase gene.
In Vivo Mammalian Alkaline Comet Assay.
In vivo carcinogenicity studies
Badanie rakotwórczości
Combined chronic toxicity/ carcinogenicity studies.
In vivo local tolerance studies
Local tolerance test.
Toxicokinetics/pharmacokinetics studies
Toxicokinetics/pharmacokinetics study.
OECD Nr 417/ EU B.436/ ICH S3A
Other services
Toxicological studies conducted according to the procedures agreed with the customer
Haematological studies
Biochemical studies
Hormone identification
Tissue and organ collection
Preparing histopathology slides and their scanning
Our specialists will answer all your questions.
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Inga Mrzyk, PhD
e-mail: inga.mrzyk@ipo.lukasiewicz.gov.plTelephone: +48 32 210 30 81 wew. 108
Ecotoxicological studies
The studies are conducted under the Good Laboratory Practice quality system 16/2023/GLP) according to methods: OECD, EU, ISO, EPA, SANTE, SANCO, SETAC, ESCORT 1, ESCORT 2, IOBC, BART, EPPO, and the results are used for registration, licensing as well as other regulations in the field of industrial chemicals.
Aquatic toxicity studies
Freshwater algae and cyanobacteria, growth inhibition test.
Lemna sp. growth inhibition test.
Sediment–free Myriophyllum spicatum toxicity test.
Water–sediment (Myriophyllum spicatum) macrophytes spicatum toxicity test.
Daphnia magna, acute immobilisation test.
Daphnia magna reproduction test.
Sediment–water chironomid toxicity using spiked sediment.
Sediment–water chironomid toxicity using spiked water.
Sediment–water chironomid life-cycle toxicity test using spiked water or spiked sediment.
Chironomus sp., acute immobilisation test.
Fish Cell Line Acute Toxicity – The RTgill-W1 cell line assay.
Fish embryo acute toxicity (FET) test.
Fish, acute toxicity test.
Fish, early-life stage toxicity test.
Fish, short-term toxicity test on embryo and sac-fry stages.
Fish, juvenile growth test.
Bioaccumulation in fish: aqueous and dietary exposure.
21-day fish assay: a short-term screening for oestrogenic and androgenic acitivity in zebrafish.
Toxicity studies for soil organisms
Terrestrial plant test, seedling emergence and seedling growth test.
Terrestrial plant test, vegetative vigour test.
Earthworm, acute toxicity test.
Earthworm, reproduction test. (Eisenia fetida/Eisenia andrei)
Collembolan, reproduction test in soil. (Folsomia candida)
Predatory mite Hypoaspis (Geolaelaps), reproduction test in soil.
Soil microorganisms: nitrogen transformation test.
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Soil microorganisms: carbon transformation test. |
Toxicity tests for pollinators and beneficial arthropods
Honeybees, acute oral toxicity test (Apis mellifera)
Honeybees, acute contact toxicity test (Apis mellifera)
Honeybees, larval toxicity test (Apis mellifera) – single exposure
Honeybees, larval toxicity test (Apis mellifera) – repeated exposure.
Honeybees (Apis mellifera), chronic oral toxicity test (10-day feeding).
Study of honeybees’ flight ability to return to the hive after a single oral exposure to sublethal doses of chemicals.
OECD Nr 332
Bumblebees (Bombus spp.), acute oral toxicity test.
Bumblebees (Bombus spp.), acute contact toxicity test.
A laboratory test or extended laboratory test for evaluating the effects of plant protection products on the parasitic wasp, Aphidius rhopalosiphi De Stephani-Perez.
SETAC; ESCORT 1, ESCORT 2; IOBC/BART/EPPO
A laboratory test or extended laboratory test for evaluating the effects of plant protection products on the predatory mite, Typhlodromus pyri Scheuten.
SETAC; ESCORT 1, ESCORT 2; IOBC/BART/EPPO
A laboratory test for evaluating the effects of plant protection products on the spider, Pardosa.
SETAC; ESCORT 1, ESCORT 2; IOBC/BART/EPPO
A laboratory test or extended laboratory test for evaluating the effects of plant protection products on the seven spotted ladybird beetle, Coccinella septempunctata L.
ESCORT 1, ESCORT 2, IOBC, BART, EPPO
A laboratory test or extended laboratory test for evaluating the effects of plant protection products on the green lacewing, Chrysoperla carnea.
ESCORT 1, ESCORT 2, IOBC, BART, EPPO
Aged residue test on the parasitic wasp, Aphidius rhopalosiphi De Stephani-Perez.
ESCORT 1, ESCORT 2, IOBC, BART, EPPO
Aged residue test on the predatory mite, Typhlodromus pyri Scheuten.
ESCORT 1, ESCORT 2, IOBC, BART, EPPO
Aged residue test on the seven spotted ladybird beetle, Coccinella septempunctata L.
ESCORT 1, ESCORT 2, IOBC, BART, EPPO
Aged residue test on the green lacewing, Chrysoperla carnea.
ESCORT 1, ESCORT 2, IOBC, BART, EPPO
A test for evaluating the chronic effects of plant protection products on the rove beetle Aleochara bilineata (Coleoptera: Staphylinidae)
SETAC; ESCORT 1, ESCORT 2; IOBC/BART/EPPO
A method for testing effects of plant protection products on the carabid beetle Poecilus cupreus (Coleoptera, Carabidae)
SETAC; ESCORT 1, ESCORT 2; IOBC/BART/EPPO
Honeybee (Apis mellifera L.) Homing Flight Test, using single oral exposure to sublethal doses of test chemical
Fate and behaviour in the environment
Ready biodegradability
OECD Nr 301D/ EU C.4-E;
OECD Nr 301F/ EU C.4-D
Inherent biodegradability: Zahn-Wellens/ EMPA test
Activated sludge, respiration inhibition test
Aerobic and anaerobic transformation in soil
Leaching in soil columns
Development and validation of an analytical method in water, soil, sediments and plant and animal materials
SANTE
Determination of the residues of chemical substances in water, soil, sedimemt, plant and animal materials
SANTE/2020/12830
Stability of pesticide residues in stored commodities
Bioaccumulation in Terrestrial Oligochaetes
Physical and chemical properties
Water solubility
Solubility in organic solvents
Adsorption – desorption using batch equilibrium method
Partition coefficient (n-octanol/water): shake flask method
Hydrolysis as a function of pH
Partition coefficient (n-octanol/water), HPLC method
Estimation of the adsorption coefficient (KOC) on soil and on sewage sludge using high performance liquid chromatography (HPLC)
Avian toxicology
Avian dietary toxicity test
Avian acute oral toxicity test
Avian repellency test (Coturnix coturnix japonica)
OECD draft
Ecosystem structure study
Simulated freshwater lentic field test (outdoor microcosms and mesocosms)
Our specialists will answer all your questions.
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Katarzyna Winiarska, MSc
e-mail: katarzyna.winiarska@ipo.lukasiewicz.gov.plTelephone: +48 32 21 03 081 ext.118
Testing for hazardous properties of materials
Testing field:
- Chemical testing (C)
- Electrical and electronical testing (E)
- Physical testing (N)
Objects:
- Chemical products
- Construction products, materials and structures
- Fuels
- Others products
- Personal protective equipment
- Textiles and leather
- Wastewater
- Fertilizers
- Paints and varnishes
- Explosives and ammunition
Our specialists will answer all your questions.
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Małgorzata Wróblewska-Piórkowska, MSc Eng.
e-mail: malgorzata.wroblewska@ipo.lukasiewicz.gov.plTelephone: +48 22 88 41 373
